Bioinformatics analysis of the AT-hook gene family in Rice Gui-Wei ZHANG
The AT-hook is a small motif existing in DNA-binding protein which was first described in the high mobility group non-histone chromosomal protein HMG-I/Y. Results show that AT-hook family proteins play an important role in chromatin structure assembly, specific binding to target cells, transcription regulation and development regulation in other organisms. In this paper, we identified 45 AT-hook genes from rice genome by bioinformatics method carried out the analysis of gene structure and chromosomal cistribution, and examined the phylogenetic relationship among rice AT-hook proteins, an unrooted tree was constructed from alignments of their full-length protein sequences in rice and Arabidopsis. These results showed that AT-hook proteins grouped into 5 distinct clades(A~E), their structures and characteristics have no significant differentiation. The family members were recruited through chromosome replication. AT-hook gene mainly expresses in young panicle. And some of the gene expression was further verified by qRT-PCR.
Keywords： AT-hook GENE, BIOINFORMATICS, GENE EXPRESSION, RICE