• 植物学报 , 2014,Vol.49(1)
  • 水稻AT-hook基因家族生物信息学分析
  • 张贵慰 曾珏 郭维 罗琼1   
  • 1. 云南农业大学 农学与生物技术学院
    2. 云南农业大学
下载: PDF     导出: TXT EndNote (RIS)

摘要:

AT-hook是一种小型的DNA结合蛋白基序,首次在哺乳动物非组蛋白染色体的高移动组蛋白(HMG-I/Y)中发现。其它生物中的研究表明,AT-hook蛋白在染色质结构组装、靶细胞特异性结合、转录调控和生长发育的调控中发挥重要作用。本文利用生物信息学方法,在水稻(Oryza sativa)基因组中鉴定出了45个编码AT-hook蛋白的基因,并对这些基因的系统进化、染色体定位,及其编码蛋白的结构、功能等进行了系统的生物信息学分析。结果表明,水稻AT-hook蛋白的结构和特性分化不显著;45个水稻AT-hook基因可划分成5个亚族;染色体复制是基因家族成员扩增的进化途径之一。基因数字表达分析结果显示,AT-hook基因主要在幼穗中表达,并通过qRT-PCR验证了部分基因的数字表达结果。

关键词: AT-hook基因,生物信息学,基因表达,水稻  

基金:

国家自然科学基金

 

 

Bioinformatics analysis of the AT-hook gene family in Rice

Gui-Wei ZHANG  

Abstract:

The AT-hook is a small motif existing in DNA-binding protein which was first described in the high mobility group non-histone chromosomal protein HMG-I/Y. Results show that AT-hook family proteins play an important role in chromatin structure assembly, specific binding to target cells, transcription regulation and development regulation in other organisms. In this paper, we identified 45 AT-hook genes from rice genome by bioinformatics method carried out the analysis of gene structure and chromosomal cistribution, and examined the phylogenetic relationship among rice AT-hook proteins, an unrooted tree was constructed from alignments of their full-length protein sequences in rice and Arabidopsis. These results showed that AT-hook proteins grouped into 5 distinct clades(A~E), their structures and characteristics have no significant differentiation. The family members were recruited through chromosome replication. AT-hook gene mainly expresses in young panicle. And some of the gene expression was further verified by qRT-PCR.

Keywords: AT-hook GENE, BIOINFORMATICS, GENE EXPRESSION, RICE